1-aminocyclopropane-1-carboxylic acid meaning in English
氨基环丙烷羧酸
Examples
- Degenerate oligonucleotides to highly conserved regions of cucumis melo 1 - aminocyclopropane - 1 - carboxylic acid ( acc ) oxidase gene were used to prime the amplification of fragment of 128bp by ploymerase chain reaction ( pcr ) in samples of genomic dna from fruit of cucumis melo l . cv hetao flesh , which was cloned into plasmid vector pmd - 18 - t . the clon of antisense orientation were selected , and it was inserted downstream of camv35s promoter and enhancer " " of tmv into the plant expression vector pbinyxw , antisence expression vector pbinya was constructed . at the base that pollination and fertilization of cucumis melo l . cv hetao was studied , using pollen tube pathway transformate cucumis melo l . cv hetao , 76 fruit had been obtained , moreover , hardness and content of sugar were analysed
本实验以河套蜜瓜果肉基因组dna为模板，用甜瓜acc氧化酶基因特异寡核苷酸链为引物进行pcr扩增，得到128bp的扩增产物。将得到的扩增产物克隆到质粒载体pmd - 18 - t上，筛选反向克隆，然后将其反向构建到植物表达载体pbinyxw的camv35s启动子和tmv增强子“ ”的下游，构建成反义表达载体pbinya 。并在对河套蜜瓜授粉受精生物学研究的基础上，通过花粉管通道法转化河套蜜瓜，共获76颗瓜，并进行了硬度和含糖量的分析。